It is important to develop a high sensitive method of determination for surfactants to study the biodegradability and aquatic toxicity of the surfactants in environment as well as its influence on the physiological process. There are many methods for determining surfactants i.e. absorption spectrophotometry, IR, electricity tension, luminosity, analytical method and HPLC.
It successfully applied to determination of low concentration of cationic surfactants in the domestic sewage and Xian city moat.