Arif
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04:55:12 pm on January 6, 2010 | # |
how spectrophotometer works
There are two major classes of devices: (1) single beam and (2) double beam. A double beam spectrophotometer compares the light intensity between two light paths, one path containing a reference sample and the other the test sample.
Wikipedia File:Spetrophotometer-en.svg
In short, the sequence of events in a spectrophotometer is as follows:
- The light source shines into a monochromator.
- A particular output wavelength is selected and beamed at the sample.
- The sample absorbs light.
intensity of that particular wavelength (I vs nm) is recorded.
